Genotyping and primer details


Genotyping and primer details for 158B07 - At1g48310
Line ID 158B07
Locus At1g48310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17853693 of line 158B07 Chr1:17853693
   
Insertion allele amplicon 8474 - AP32
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AP32: 5'-ATTAGGATGCTCAAGCTGGTTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17854105
   
Insertion allele amplicon 8474 - NB28
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NB28: 5'-TACCAAGTATTTCCCCACAAAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17853161
   
wt amplicon (over InsPos) AP32 - NB28
wt amplicon start Chr1:17853161
wt amplicon end Chr1:17854105
wt amplicon size 945 bp
Locus specific primer NB28: 5'-TACCAAGTATTTCCCCACAAAGAC-3'
2nd locus specific primer AP32: 5'-ATTAGGATGCTCAAGCTGGTTAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11