Genotyping and primer details


Genotyping and primer details for 161G03 - At1g51490
Line ID 161G03
Locus At1g51490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19096671 of line 161G03 Chr1:19096671
   
Insertion allele amplicon 8474 - AN05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AN05: 5'-ACTGACTGTGAATTTGGAGGAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19096095
   
Insertion allele amplicon 8474 - VL17
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer VL17: 5'-GAGTGTAACCATTTCCGAATTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19097357
   
wt amplicon (over InsPos) AF04 - AN05
wt amplicon start Chr1:19096095
wt amplicon end Chr1:19097004
wt amplicon size 910 bp
Locus specific primer AN05: 5'-ACTGACTGTGAATTTGGAGGAGAT-3'
2nd locus specific primer AF04: 5'-CGGTGATGTAGGTTAATGGATTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11