Genotyping and primer details


Genotyping and primer details for 164F12 - At2g46270
Line ID 164F12
Locus At2g46270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19002694 of line 164F12 Chr2:19002694
   
Insertion allele amplicon 8474 - AJ16
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AJ16: 5'-AACAATTGGTTCAAGCTAGCTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19002061
   
Insertion allele amplicon 8474 - AR10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AR10: 5'-CATAACACCAAATTTTCATCTGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19003104
   
Insertion allele amplicon 8474 - Z55b
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer Z55b: 5'-TCATGTCCCAAAGAGTTAGAATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19001983
   
wt amplicon (over InsPos) AR10 - AJ16
wt amplicon start Chr2:19002061
wt amplicon end Chr2:19003104
wt amplicon size 1044 bp
Locus specific primer AJ16: 5'-AACAATTGGTTCAAGCTAGCTCAT-3'
2nd locus specific primer AR10: 5'-CATAACACCAAATTTTCATCTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11