Genotyping and primer details


Genotyping and primer details for 166E01 - At1g77130
Line ID 166E01
Locus At1g77130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28980026 of line 166E01 Chr1:28980026
   
Insertion allele amplicon 8474 - AK05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AK05: 5'-AATGTTGAATTAGATGGCTCAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28979661
   
wt amplicon (over InsPos) 22dy - AK05
wt amplicon start Chr1:28979661
wt amplicon end Chr1:28980588
wt amplicon size 928 bp
Locus specific primer AK05: 5'-AATGTTGAATTAGATGGCTCAACC-3'
2nd locus specific primer 22dy: 5'-GCAAAGAACATAACATGGGAATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11