Genotyping and primer details


Genotyping and primer details for 168A03 - At5g58850
Line ID 168A03
Locus At5g58850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23765505 of line 168A03 Chr5:23765505
   
Insertion allele amplicon 8474 - M119
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer M119: 5'-ACATTAACAAGGACAATGACGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23764882
   
wt amplicon (over InsPos) 21fo - M119
wt amplicon start Chr5:23764882
wt amplicon end Chr5:23765886
wt amplicon size 1005 bp
Locus specific primer M119: 5'-ACATTAACAAGGACAATGACGAGA-3'
2nd locus specific primer 21fo: 5'-TATATGCACTTTTTGTGCCTTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11