Genotyping and primer details


Genotyping and primer details for 170E12 - At3g15690
Line ID 170E12
Locus At3g15690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5317206 of line 170E12 Chr3:5317206
   
Insertion allele amplicon 8474 - LB17
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer LB17: 5'-AAATCAGCCAAAACAAGACAGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5317728
   
wt amplicon (over InsPos) 20gl - LB17
wt amplicon start Chr3:5316860
wt amplicon end Chr3:5317728
wt amplicon size 869 bp
Locus specific primer LB17: 5'-AAATCAGCCAAAACAAGACAGAGT-3'
2nd locus specific primer 20gl: 5'-CTATAAAGGCCCAAATGAAACAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11