Genotyping and primer details


Genotyping and primer details for 172B09 - At5g07580
Line ID 172B09
Locus At5g07580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2400205 of line 172B09 Chr5:2400205
   
Insertion allele amplicon 8474 - MZ02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MZ02: 5'-GGTTGTTTAGAGACCTGGAAACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2400541
   
wt amplicon (over InsPos) 01au - MZ02
wt amplicon start Chr5:2399752
wt amplicon end Chr5:2400541
wt amplicon size 790 bp
Locus specific primer MZ02: 5'-GGTTGTTTAGAGACCTGGAAACTG-3'
2nd locus specific primer 01au: 5'-TTTGGAAGCTATACAGAGCCATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11