Genotyping and primer details


Genotyping and primer details for 175G10 - At2g20230
Line ID 175G10
Locus At2g20230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8727352 of line 175G10 Chr2:8727352
   
Insertion allele amplicon 8474 - EO04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EO04: 5'-ATGCTCGAATCTTGTTTCTTGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8726772
   
wt amplicon (over InsPos) 17ee - EO04
wt amplicon start Chr2:8726772
wt amplicon end Chr2:8727791
wt amplicon size 1020 bp
Locus specific primer EO04: 5'-ATGCTCGAATCTTGTTTCTTGTTT-3'
2nd locus specific primer 17ee: 5'-TCGATCTTATCTGATGAAACAACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11