Genotyping and primer details


Genotyping and primer details for 176H03 - At3g55120
Line ID 176H03
Locus At3g55120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20430445 of line 176H03 Chr3:20430445
   
Insertion allele amplicon 3144 - 36vq
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 36vq: 5'-ACCTGTCTGACTCATCACAACAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20429694
   
Insertion allele amplicon 8474 - MR28
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MR28: 5'-GTGGCTATATGGAAACAATTAGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20430858
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11