Genotyping and primer details


Genotyping and primer details for 179G11 - At5g61840
Line ID 179G11
Locus At5g61840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24840160 of line 179G11 Chr5:24840160
   
Insertion allele amplicon 8474 - SS11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer SS11: 5'-TCATCCACAAACACACCAATATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24839624
   
wt amplicon (over InsPos) 16au - SS11
wt amplicon start Chr5:24839624
wt amplicon end Chr5:24840377
wt amplicon size 754 bp
Locus specific primer SS11: 5'-TCATCCACAAACACACCAATATCT-3'
2nd locus specific primer 16au: 5'-GATTTGTGCCTGTGATACTGCTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11