Genotyping and primer details

Genotyping and primer details for 180A11 - At5g06780
Line ID 180A11
Locus At5g06780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2094045 of line 180A11 Chr5:2094045
Insertion allele amplicon 8474 - BU02
Locus specific primer BU02: 5'-GATAAGGCCAAGAAGCTACTCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2094467
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 14:50