Genotyping and primer details


Genotyping and primer details for 192F07 - At3g05260
Line ID 192F07
Locus At3g05260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1498913 of line 192F07 Chr3:1498913
   
Insertion allele amplicon 8474 - KT57
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KT57: 5'-ATCTGGTTACAAACGTGATGAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1499362
   
wt amplicon (over InsPos) 02bx - KT57
wt amplicon start Chr3:1498582
wt amplicon end Chr3:1499362
wt amplicon size 781 bp
Locus specific primer KT57: 5'-ATCTGGTTACAAACGTGATGAACA-3'
2nd locus specific primer 02bx: 5'-GCTTTACCTATCCCTGAGTCTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11