Genotyping and primer details


Genotyping and primer details for 211B01 - At4g38900
Line ID 211B01
Locus At4g38900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18141178 of line 211B01 Chr4:18141178
   
Insertion allele amplicon 8474 - DB01
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DB01: 5'-AAGCTTGATCTTTTAGGGGTTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18141595
   
wt amplicon (over InsPos) 09gy - DB01
wt amplicon start Chr4:18140731
wt amplicon end Chr4:18141595
wt amplicon size 865 bp
Locus specific primer DB01: 5'-AAGCTTGATCTTTTAGGGGTTTTC-3'
2nd locus specific primer 09gy: 5'-TGCTCTCCATATCATCCCTATTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11