Genotyping and primer details


Genotyping and primer details for 227H02 - At3g16940
Line ID 227H02
Locus At3g16940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5783689 of line 227H02 Chr3:5783689
   
Insertion allele amplicon 8474 - 21ii
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21ii: 5'-CTTTTCCATTTTGTAATCCAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5784098
   
wt amplicon (over InsPos) 14ir - 21ii
wt amplicon start Chr3:5783315
wt amplicon end Chr3:5784098
wt amplicon size 784 bp
Locus specific primer 21ii: 5'-CTTTTCCATTTTGTAATCCAAACC-3'
2nd locus specific primer 14ir: 5'-AGTTATGAATGGATCAGGGTGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11