Genotyping and primer details


Genotyping and primer details for 228B12 - At1g18570
Line ID 228B12
Locus At1g18570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6390212 of line 228B12 Chr1:6390212
   
Insertion allele amplicon 3144 - 47xd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 47xd: 5'-GCAATCTCATATTCGGATGGATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6391339
   
Insertion allele amplicon 8474 - RS05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RS05: 5'-TTTTTGTCCCTCTTTGTTTCACTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6389688
   
wt amplicon (over InsPos) CB36 - RS05
wt amplicon start Chr1:6389688
wt amplicon end Chr1:6390456
wt amplicon size 769 bp
Locus specific primer RS05: 5'-TTTTTGTCCCTCTTTGTTTCACTT-3'
2nd locus specific primer CB36: 5'-GTTCTTCTTCGCCTTGTCATTATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11