Genotyping and primer details


Genotyping and primer details for 242E12 - At2g14880
Line ID 242E12
Locus At2g14880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:6394805 of line 242E12 Chr2:6394805
   
Insertion allele amplicon 8474 - TS02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer TS02: 5'-AGCGAAACAAAACCATAAGAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6394463
   
wt amplicon (over InsPos) 10id - TS02
wt amplicon start Chr2:6394463
wt amplicon end Chr2:6395395
wt amplicon size 933 bp
Locus specific primer TS02: 5'-AGCGAAACAAAACCATAAGAGAAA-3'
2nd locus specific primer 10id: 5'-ATTGGTGTGGTTCTATGGTTTAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11