Genotyping and primer details


Genotyping and primer details for 244G02 - At1g05100
Line ID 244G02
Locus At1g05100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1470368 of line 244G02 Chr1:1470368
   
Insertion allele amplicon 8474 - DL86
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DL86: 5'-CTTCTTGGGTTGGAGTACATTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1470021
   
wt amplicon (over InsPos) 20dw - DL86
wt amplicon start Chr1:1470021
wt amplicon end Chr1:1470737
wt amplicon size 717 bp
Locus specific primer DL86: 5'-CTTCTTGGGTTGGAGTACATTCAT-3'
2nd locus specific primer 20dw: 5'-AATTGAATCGAATAATCAACGCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11