Genotyping and primer details


Genotyping and primer details for 272B11 - At3g07720
Line ID 272B11
Locus At3g07720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2466446 of line 272B11 Chr3:2466446
   
Insertion allele amplicon 8474 - CQ17
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CQ17: 5'-ACTGATGGAGGAGGGTTTAATTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2465827
   
wt amplicon (over InsPos) 02mj - CQ17
wt amplicon start Chr3:2465827
wt amplicon end Chr3:2466759
wt amplicon size 933 bp
Locus specific primer CQ17: 5'-ACTGATGGAGGAGGGTTTAATTTT-3'
2nd locus specific primer 02mj: 5'-ATCAGTATCTGTTTCCCAACAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11