Genotyping and primer details


Genotyping and primer details for 277E09 - At1g07150
Line ID 277E09
Locus At1g07150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2194607 of line 277E09 Chr1:2194607
   
Insertion allele amplicon 8474 - AJ19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AJ19: 5'-TTAAATGCTCCAAAATCCGAATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2194143
   
Insertion allele amplicon 8474 - DL76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DL76: 5'-CTCTCAGTGTCACAACTCATCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2194942
   
wt amplicon (over InsPos) AJ19 - DL76
wt amplicon start Chr1:2194143
wt amplicon end Chr1:2194942
wt amplicon size 800 bp
Locus specific primer DL76: 5'-CTCTCAGTGTCACAACTCATCTCC-3'
2nd locus specific primer AJ19: 5'-TTAAATGCTCCAAAATCCGAATAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11