Genotyping and primer details


Genotyping and primer details for 285E09 - At2g46510
Line ID 285E09
Locus At2g46510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19092145 of line 285E09 Chr2:19092145
   
Insertion allele amplicon 8474 - MH08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MH08: 5'-TGAGGAAGAGGAATCAAAAGTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19092603
   
wt amplicon (over InsPos) 13lb - MH08
wt amplicon start Chr2:19091742
wt amplicon end Chr2:19092603
wt amplicon size 862 bp
Locus specific primer MH08: 5'-TGAGGAAGAGGAATCAAAAGTTGT-3'
2nd locus specific primer 13lb: 5'-TCTCTTTCTCGGTCTCTTCTCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11