Genotyping and primer details


Genotyping and primer details for 328G05 - At5g38710
Line ID 328G05
Locus At5g38710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15502055 of line 328G05 Chr5:15502055
   
Insertion allele amplicon 8474 - NS11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NS11: 5'-TTGCGTGATTAACGTGTTAACTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15502596
   
wt amplicon (over InsPos) 17hf - NS11
wt amplicon start Chr5:15501624
wt amplicon end Chr5:15502596
wt amplicon size 973 bp
Locus specific primer NS11: 5'-TTGCGTGATTAACGTGTTAACTTT-3'
2nd locus specific primer 17hf: 5'-AGTACTCCGTATCGTGAAAGGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11