Genotyping and primer details


Genotyping and primer details for 365G02 - At5g10740
Line ID 365G02
Locus At5g10740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3395164 of line 365G02 Chr5:3395164
   
Insertion allele amplicon 8474 - PC82
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PC82: 5'-TATTGGTGGTGCCATACAAAGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3394735
   
Insertion allele amplicon 8474 - PC83
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PC83: 5'-TGTTCGATTCGATTTATCTCACTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3395644
   
wt amplicon (over InsPos) PC83 - PC82
wt amplicon start Chr5:3394735
wt amplicon end Chr5:3395644
wt amplicon size 910 bp
Locus specific primer PC82: 5'-TATTGGTGGTGCCATACAAAGATA-3'
2nd locus specific primer PC83: 5'-TGTTCGATTCGATTTATCTCACTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11