Genotyping and primer details


Genotyping and primer details for 377E12 - At3g29035
Line ID 377E12
Locus At3g29035 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:11035162 of line 377E12 Chr3:11035162
   
Insertion allele amplicon 8474 - NS35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NS35: 5'-TTTTTCATACACGGACTGATGGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:11034561
   
Insertion allele amplicon 8474 - NS36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NS36: 5'-AAAGCCCAAAATATCAACAAACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:11035830
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11