Genotyping and primer details


Genotyping and primer details for 399F01 - At4g03390
Line ID 399F01
Locus At4g03390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1491119 of line 399F01 Chr4:1491119
   
Insertion allele amplicon 8474 - 06cj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06cj: 5'-TCAAAGAATCGAGTAACACACCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1490572
   
Insertion allele amplicon 8474 - 40wq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 40wq: 5'-GAATTCAAGAAGAAACTTTCGTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1491858
   
Insertion allele amplicon 8474 - EQ49
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ49: 5'-CAACACAGAGCAAGTTCTATGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1490524
   
wt amplicon (over InsPos) 06cj - EQ50
wt amplicon start Chr4:1490572
wt amplicon end Chr4:1491553
wt amplicon size 982 bp
Locus specific primer EQ50: 5'-CAATGAGCTTGATTCTGATGGATA-3'
2nd locus specific primer 06cj: 5'-TCAAAGAATCGAGTAACACACCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11