Genotyping and primer details


Genotyping and primer details for 402G03 - At2g21990
Line ID 402G03
Locus At2g21990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9360085 of line 402G03 Chr2:9360085
   
Insertion allele amplicon 8474 - JG82
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JG82: 5'-CTCTGAATCTTTAGACCCAACGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9359642
   
wt amplicon (over InsPos) 51dy - JG82
wt amplicon start Chr2:9359642
wt amplicon end Chr2:9360673
wt amplicon size 1032 bp
Locus specific primer JG82: 5'-CTCTGAATCTTTAGACCCAACGAC-3'
2nd locus specific primer 51dy: 5'-ATTTTTGGAAACTCGTTTGATTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11