Genotyping and primer details


Genotyping and primer details for 426E07 - At1g11560
Line ID 426E07
Locus At1g11560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3882807 of line 426E07 Chr1:3882807
   
Insertion allele amplicon 8474 - 32lm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32lm: 5'-AGAAGGTTTCTTGTGGGTCAAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3883114
   
wt amplicon (over InsPos) 02mr - 32lm
wt amplicon start Chr1:3882340
wt amplicon end Chr1:3883114
wt amplicon size 775 bp
Locus specific primer 32lm: 5'-AGAAGGTTTCTTGTGGGTCAAGTA-3'
2nd locus specific primer 02mr: 5'-CTCATATCAAAGACACAAATCGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11