Genotyping and primer details


Genotyping and primer details for 445E03 - At5g52600
Line ID 445E03
Locus At5g52600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21344101 of line 445E03 Chr5:21344101
   
Insertion allele amplicon 3144 - FS08
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FS08: 5'-GATCAGTGGTGAAAGAGGAAAGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21343589
   
Insertion allele amplicon 8474 - HV15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HV15: 5'-TGAGCCAAACAAAAGTTACACAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21344710
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11