Genotyping and primer details


Genotyping and primer details for 453H07 - At2g20110
Line ID 453H07
Locus At2g20110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8685861 of line 453H07 Chr2:8685861
   
Insertion allele amplicon 8474 - 57kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 57kh: 5'-TCTTTCTGATTTGCTAACCGTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8686185
   
wt amplicon (over InsPos) 04ky - 57kh
wt amplicon start Chr2:8685541
wt amplicon end Chr2:8686185
wt amplicon size 645 bp
Locus specific primer 57kh: 5'-TCTTTCTGATTTGCTAACCGTTTT-3'
2nd locus specific primer 04ky: 5'-CAAGCAAACATTCTTTGTTCTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11