Genotyping and primer details


Genotyping and primer details for 475C05 - At2g35940
Line ID 475C05
Locus At2g35940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15089287 of line 475C05 Chr2:15089287
   
Insertion allele amplicon 8474 - JU19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JU19: 5'-CTAAGCAAACAGGACTCACTCGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15090034
   
Insertion allele amplicon 8474 - JU20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JU20: 5'-ATACACCCAATGCAAGCTATTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15088926
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11