Genotyping and primer details


Genotyping and primer details for 480C06 - At5g57180
Line ID 480C06
Locus At5g57180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23169686 of line 480C06 Chr5:23169686
   
Insertion allele amplicon 8474 - PB05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PB05: 5'-ATTTTCTGTTTCATCCAACGATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23168772
   
wt amplicon (over InsPos) 06jz - PB05
wt amplicon start Chr5:23168772
wt amplicon end Chr5:23170208
wt amplicon size 1437 bp
Locus specific primer PB05: 5'-ATTTTCTGTTTCATCCAACGATTC-3'
2nd locus specific primer 06jz: 5'-GAGTTCGACGTTTCTCTTTGTACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11