Genotyping and primer details


Genotyping and primer details for 516B03 - At3g14030
Line ID 516B03
Locus At3g14030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4646734 of line 516B03 Chr3:4646734
   
Insertion allele amplicon 8474 - NG54
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NG54: 5'-TCAGTTGTATGGAATCCTTGTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4647098
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11