Genotyping and primer details


Genotyping and primer details for 516G02 - At4g02880
Line ID 516G02
Locus At4g02880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1278717 of line 516G02 Chr4:1278717
   
Insertion allele amplicon 8474 - 11wq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11wq: 5'-TCTTTGTCAAGACGTTGACTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1279201
   
Insertion allele amplicon 8474 - 29wq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29wq: 5'-TCCAAGCGATTAATCTCAGTTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1279700
   
Insertion allele amplicon 8474 - PF15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PF15: 5'-AATTAGAGCTTCACAATTCTCGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1278225
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11