Genotyping and primer details


Genotyping and primer details for 593C11 - At4g36180
Line ID 593C11
Locus At4g36180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17122861 of line 593C11 Chr4:17122861
   
Insertion allele amplicon 8474 - EU12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU12: 5'-TCCGTCTTTCTTCAGTACAACTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17123341
   
wt amplicon (over InsPos) ST03 - EU12
wt amplicon start Chr4:17122431
wt amplicon end Chr4:17123341
wt amplicon size 911 bp
Locus specific primer EU12: 5'-TCCGTCTTTCTTCAGTACAACTCA-3'
2nd locus specific primer ST03: 5'-GACGAAGGAACATAACCTGAGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11