Genotyping and primer details


Genotyping and primer details for 625G03 - At3g09620
Line ID 625G03
Locus At3g09620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2950510 of line 625G03 Chr3:2950510
   
Insertion allele amplicon 8474 - NG70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NG70: 5'-CAAGGATATCTCAAGGATGACACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2951114
   
wt amplicon (over InsPos) 15sl - NG70
wt amplicon start Chr3:2950193
wt amplicon end Chr3:2951114
wt amplicon size 922 bp
Locus specific primer NG70: 5'-CAAGGATATCTCAAGGATGACACA-3'
2nd locus specific primer 15sl: 5'-AGATAGACAGGCTCATTTGGTCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11