Genotyping and primer details


Genotyping and primer details for 640E09 - At1g03040
Line ID 640E09
Locus At1g03040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:704957 of line 640E09 Chr1:704957
   
Insertion allele amplicon 8474 - 09ih
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09ih: 5'-TAATGGCATTTCAGTGACTAGTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:704603
   
wt amplicon (over InsPos) 13ji - 09ih
wt amplicon start Chr1:704603
wt amplicon end Chr1:705408
wt amplicon size 806 bp
Locus specific primer 09ih: 5'-TAATGGCATTTCAGTGACTAGTGG-3'
2nd locus specific primer 13ji: 5'-GAACGGTAAGACCTAACCTGAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11