Genotyping and primer details


Genotyping and primer details for 651G08 - At1g63430
Line ID 651G08
Locus At1g63430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23524253 of line 651G08 Chr1:23524253
   
Insertion allele amplicon 8474 - EU32
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU32: 5'-GTTAATCTCGAAACATCCTTCAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23524956
   
wt amplicon (over InsPos) SP07 - EU32
wt amplicon start Chr1:23523902
wt amplicon end Chr1:23524956
wt amplicon size 1055 bp
Locus specific primer EU32: 5'-GTTAATCTCGAAACATCCTTCAGC-3'
2nd locus specific primer SP07: 5'-ATTCATCTTTTTCGCAGTCAAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11