Genotyping and primer details


Genotyping and primer details for 653B01 - At2g30040
Line ID 653B01
Locus At2g30040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12822802 of line 653B01 Chr2:12822802
   
Insertion allele amplicon 8474 - 08ah
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08ah: 5'-CAACTTCACGTTGACACTAACCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12823356
   
Insertion allele amplicon 8474 - RU83
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer RU83: 5'-GGAGGTGGTTAGAAGAGAGTACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12822298
   
wt amplicon (over InsPos) 08ah - RU83
wt amplicon start Chr2:12822298
wt amplicon end Chr2:12823356
wt amplicon size 1059 bp
Locus specific primer RU83: 5'-GGAGGTGGTTAGAAGAGAGTACCA-3'
2nd locus specific primer 08ah: 5'-CAACTTCACGTTGACACTAACCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11