Genotyping and primer details


Genotyping and primer details for 655B10 - At3g07880
Line ID 655B10
Locus At3g07880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2514027 of line 655B10 Chr3:2514027
   
Insertion allele amplicon 3144 - AG10
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AG10: 5'-GGTAGCACCTATTGTTTGATTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2513648
   
Insertion allele amplicon 8474 - HP80
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP80: 5'-AAGAAGAAGCAAAAAGGAGAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2514431
   
wt amplicon (over InsPos) HP80 - AG10
wt amplicon start Chr3:2513648
wt amplicon end Chr3:2514431
wt amplicon size 784 bp
Locus specific primer AG10: 5'-GGTAGCACCTATTGTTTGATTGGT-3'
2nd locus specific primer HP80: 5'-AAGAAGAAGCAAAAAGGAGAAACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11