Genotyping and primer details


Genotyping and primer details for 659G11 - At1g23870
Line ID 659G11
Locus At1g23870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8434092 of line 659G11 Chr1:8434092
   
Insertion allele amplicon 8474 - FQ79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FQ79: 5'-CAAACTTATAGCCATGGAACGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8433747
   
wt amplicon (over InsPos) 09bj - FQ79
wt amplicon start Chr1:8433747
wt amplicon end Chr1:8434587
wt amplicon size 841 bp
Locus specific primer FQ79: 5'-CAAACTTATAGCCATGGAACGTCT-3'
2nd locus specific primer 09bj: 5'-CACAGTGTTTTTAGGATCTCCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11