Genotyping and primer details


Genotyping and primer details for 671E09 - At3g12100
Line ID 671E09
Locus At3g12100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3857296 of line 671E09 Chr3:3857296
   
Insertion allele amplicon 8474 - GG27
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GG27: 5'-TAGTTTTTGAGAGAGAAACGGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3857881
   
Insertion allele amplicon 8474 - NC34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NC34: 5'-TGGTGGAAACAGCTGTCTTATTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3857711
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11