Genotyping and primer details


Genotyping and primer details for 692A05 - At2g31320
Line ID 692A05
Locus At2g31320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13355373 of line 692A05 Chr2:13355373
   
Insertion allele amplicon CR3S - 85vr
T-DNA primer CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Locus specific primer 85vr: 5'-ATGTATTTCTCCCAATGCAACTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13354653
   
Insertion allele amplicon 8474 - BE77
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BE77: 5'-GTCTTCTTCTCCTCTTCTCTCCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13355981
   
Insertion allele amplicon CR3S - FN52
T-DNA primer CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Locus specific primer FN52: 5'-TAGGAGCATCTTATTGCCAAGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13355109
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11