Genotyping and primer details


Genotyping and primer details for 697G04 - At1g65670
Line ID 697G04
Locus At1g65670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24423672 of line 697G04 Chr1:24423672
   
Insertion allele amplicon 8474 - NE14
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NE14: 5'-AGACAACCTCTCCTAGCTCCTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24423366
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11