Genotyping and primer details


Genotyping and primer details for 700B03 - At1g32530
Line ID 700B03
Locus At1g32530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11762250 of line 700B03 Chr1:11762250
   
Insertion allele amplicon 8474 - BC31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BC31: 5'-CTAGAGTACCACCCGTACCAACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11761702
   
wt amplicon (over InsPos) BC32 - BC31
wt amplicon start Chr1:11761702
wt amplicon end Chr1:11762627
wt amplicon size 926 bp
Locus specific primer BC31: 5'-CTAGAGTACCACCCGTACCAACAT-3'
2nd locus specific primer BC32: 5'-TCTCTCTCTCTCTCTCTCTCGCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11