Genotyping and primer details


Genotyping and primer details for 751B12 - At1g72650
Line ID 751B12
Locus At1g72650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27351908 of line 751B12 Chr1:27351908
   
Insertion allele amplicon 8474 - BE46
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BE46: 5'-GAGCCATAATGTTCTCTCTTGGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27352303
   
wt amplicon (over InsPos) 08dw - BE46
wt amplicon start Chr1:27351560
wt amplicon end Chr1:27352303
wt amplicon size 744 bp
Locus specific primer BE46: 5'-GAGCCATAATGTTCTCTCTTGGAC-3'
2nd locus specific primer 08dw: 5'-ATGAAGCTTTACTGCAGGATGAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11