Genotyping and primer details


Genotyping and primer details for 764D11 - At1g35520
Line ID 764D11
Locus At1g35520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:13084211 of line 764D11 Chr1:13084211
   
Insertion allele amplicon 8474 - 60wl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 60wl: 5'-GCTAATGGTGTATTTTCTGTTCCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13085003
   
Insertion allele amplicon 8474 - KQ05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KQ05: 5'-GCATCTTCTGTTGTTTCACGATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13083527
   
Insertion allele amplicon 8474 - KQ06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KQ06: 5'-GTTTTAGGGGAGAGACTGGAGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13109972
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11