Genotyping and primer details


Genotyping and primer details for 787G04 - At4g32950
Line ID 787G04
Locus At4g32950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15905825 of line 787G04 Chr4:15905825
   
Insertion allele amplicon 8474 - 07ah
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07ah: 5'-AGTAGTTCCAGAAGCAGAGCAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15905503
   
Insertion allele amplicon 8474 - CN08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CN08: 5'-TTTCTAATATAATTGGCGGAAACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15906367
   
wt amplicon (over InsPos) 07ah - CN08
wt amplicon start Chr4:15905503
wt amplicon end Chr4:15906367
wt amplicon size 865 bp
Locus specific primer CN08: 5'-TTTCTAATATAATTGGCGGAAACG-3'
2nd locus specific primer 07ah: 5'-AGTAGTTCCAGAAGCAGAGCAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11