Genotyping and primer details


Genotyping and primer details for 799H09 - At5g13180
Line ID 799H09
Locus At5g13180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4197479 of line 799H09 Chr5:4197479
   
Insertion allele amplicon 8474 - DB27
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DB27: 5'-CTCTAGAGGAAATCAAATCGTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4197045
   
wt amplicon (over InsPos) 20km - DB27
wt amplicon start Chr5:4197045
wt amplicon end Chr5:4197913
wt amplicon size 869 bp
Locus specific primer DB27: 5'-CTCTAGAGGAAATCAAATCGTTGG-3'
2nd locus specific primer 20km: 5'-ATATTTTCCGTTTGTGGTAGCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11