Genotyping and primer details


Genotyping and primer details for 821C08 - At1g74160
Line ID 821C08
Locus At1g74160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27888615 of line 821C08 Chr1:27888615
   
Insertion allele amplicon 8474 - 37dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 37dl: 5'-CTTATCAAACGAGCTGGTACATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27889422
   
wt amplicon (over InsPos) 24ep - 37dl
wt amplicon start Chr1:27888265
wt amplicon end Chr1:27889422
wt amplicon size 1158 bp
Locus specific primer 37dl: 5'-CTTATCAAACGAGCTGGTACATGA-3'
2nd locus specific primer 24ep: 5'-GTTAGACAGAGCAGACGAGAGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11