Genotyping and primer details


Genotyping and primer details for 836G11 - At5g02470
Line ID 836G11
Locus At5g02470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:543349 of line 836G11 Chr5:543349
   
Insertion allele amplicon 3144 - FP62
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FP62: 5'-AAATCTACGTTTGAGACTGTGGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:543826
   
Insertion allele amplicon 8474 - JL23
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL23: 5'-ACTACTGCGTGAGGGTTTGTCTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:542908
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11