Genotyping and primer details


Genotyping and primer details for 848H04 - At1g18320
Line ID 848H04
Locus At1g18320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6304468 of line 848H04 Chr1:6304468
   
Insertion allele amplicon 8474 - PF79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PF79: 5'-TCACACAGAATGTGAACAAATCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6304903
   
wt amplicon (over InsPos) 01ul - PF79
wt amplicon start Chr1:6303991
wt amplicon end Chr1:6304903
wt amplicon size 913 bp
Locus specific primer PF79: 5'-TCACACAGAATGTGAACAAATCAA-3'
2nd locus specific primer 01ul: 5'-TGATCTCTGTCTTTGGTTGTTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11